Probiotic, Antimicrobial and Bioactive Properties of Lactic Acid Bacteria Isolated from Goat Milk
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- January 31, 2019
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Mary Tomi Olorunkosebi1, Bukola Titilayo Fagbemi2, Samuel Olalekan Oyetunji3 & Afeez Adesina Adedayo4
1 4Department of Biological Sciences, Western Illinois University, Macomb, Illinois, USA
2Department of Health Sciences, Western Illinois University, Macomb, Illinois, USA
3Department of English, University of Ibadan, Ibadan, Oyo State, Nigeria
This study aimed at isolating and identifying lactic acid bacteria (LAB) from goat milk and the determination of their probiotic, antimicrobial and antioxidant potential. Raw milk samples were collected into sterile corked bottles from Sokoto Red and the West African dwarf goats and transported in ice packs to the laboratory. LAB were isolated and identified following standard techniques and were subjected to different probiotic assays including bile salt and acid tolerance. Their lipolytic and proteolytic activities, their antimicrobial activity against selected pathogens, and their capability to produce lactic acid, diacetyl and hydrogen peroxide were also determined. Safety assessment tests to determine if they can produce haemolysin, gelatinase, DNase and lecithinase were also performed. Their bioactive potential was assessed. The isolates were finally subjected to a number of antibiotics. A total of 34 LAB strains were isolated and were identified to belong to 10 species namely; Lactobacillus plantarum (26.5%), Lactobacillus casei (14.7%), Pediococcus spp. (8.8%), Streptococcus thermophilus (8.8%), Lactobacillus acidophilus (8.8%), Lactobacillus helviticus (14.7%), Lactobacillus brevis (5.9%), Lactobacillus delbrueckii (5.9%), Lactobacillus bulgaricus (2.9%) and Lactobacillus fermentum (2.9%). Of all the 34 LAB isolates, only two exhibited desirable probiotic, antimicrobial and antioxidant properties. These two LAB isolates are useful candidates as starter cultures in food fermentation.
Keywords: Goat Milk; Lactic Acid Bacteria (LAB); Probiotic; Antimicrobial; Bioactive Properties
Citations: Olorunkosebi, M. A., Fagbemi, B. T., Oyetunji, S. O. & Adebayo, A. A. (2019). Probiotic, Antimicrobial and Bioactive Properties of Lactic Acid Bacteria Isolated from Goat Milk. Global Journal of Biology and Life Sciences, 1(2) 1-19 https://doi.org/10.5281/zenodo.18784179